TABLE 1.
List of antibodies and applications.
| Primary antibodies | Dilution (WB/IF) | Clone and/or reference code | Supplier | |
| 53BP1 | Rabbit polyclonal | -; 1/200 | H300, sc-22760 | Santa Cruz Biotechnology |
| 53BP1 | Rabbit polyclonal | 1/500; 1/200 | NB100-304 | Novus Biologicals |
| γH2AX | Mouse monoclonal | -; 1/200 | Clone JBW301; 05-636 | Millipore |
| MDC1 | Rabbit polyclonal | 1/500; 1/200 | ab11169 | Abcam |
| NBS1 | Mouse monoclonal | -; 1/200 | 611871 | BD Biosciences |
| NBS1 (Nibrin) | Rabbit polyclonal | 1/1000; - | N 3162 | Sigma-Aldrich |
| PARP1 | Mouse monoclonal | 1/1000; - | sc-8007 | Santa Cruz Biotechnology |
| β-actin | Mouse monoclonal | 1/2000; - | AC15/A5441 | Sigma-Aldrich |
| Histone H4-K16ac | Rabbit monoclonal | 1/500; 1/400 | ab109463 | Abcam |
| Histone H4-K20me2 | Rabbit polyclonal | 1/500; 1/100 | 9759 | Cell Signaling |
| H3 | Rabbit polyclonal | 1/1000; - | 9175 | Cell Signaling |
| Cleaved caspase 3 | Rabbit monoclonal | 1/1000; - | 5A1E/9664 | Cell Signaling |
| Secondary antibodies | Dilution (WB/IF) | Reference code | Supplier | |
| Anti-mouse IgG (WB) | Goat Anti-Mouse IgG, DyLight 680 (red) | 1/10000; - | 35518 | Thermo Scientific |
| Anti-rabbit IgG (WB) | Goat Anti-Rabbit IgG, DyLight 800 (green) | 1/10000; - | 35571 | Thermo Scientific |
| Goat anti-Mouse IgG (IF) | Goat anti-Mouse IgG linked to Cy3 (red) | -; 1/1000 | 115-165-146 | Jackson ImmunoResearch |
| Goat anti-rabbit IgG (IF) | Goat anti-rabbit IgG linked to Cy2 (green) | -; 1/1000 | 111-225-144 | Jackson ImmunoResearch |