FIG. 2.

SDS-PAGE and Western blot of the expressed products of the three P37 gene constructs. (A) SDS-PAGE protein profiles stained with Coomassie brilliant blue; (B) Western blot of the gel depicted in panel A probed with monospecific anti-P37. Lanes: Bb, B. burgdorferi B31 low-passage whole-cell lysate; E. coli lysates, IPTG-induced lysates containing the pSCREEN plasmid vector only and constructs F1, F2, and F3. Protein standards with molecular sizes in kilodaltons are shown to the left of panel A.