FIG. 3.

IgM Western blots of serum samples against B. burgdorferi lysate (A) and against recombinant P37 expressed in an E. coli lysate (B). Lanes M contain marker antibody reflecting the position of the P37 antigen. Arrows indicate the positions of the P37 bands and the B. burgdorferi 41- and 39-kDa doublet bands. Lanes: 1 to 13, serum samples with positive reactivity to the B. burgdorferi P37 (samples in lanes 10 and 12 were reactive to recombinant P37 but the reaction was weaker); 14 to 19, serum samples from patients with early Lyme disease with no observed immunoreactivity against B. burgdorferi P37.