Skip to main content
. Author manuscript; available in PMC: 2022 Sep 14.
Published in final edited form as: Immunity. 2021 Aug 2;54(9):1948–1960.e5. doi: 10.1016/j.immuni.2021.07.001

KEY RESOURCES TABLE.

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
mouse monoclonal anti-β Actin SIGMA cat # A5441
ADAR1 Antibody (15.8.6) Santa Cruz Biotechnology cat # sc-73408
Bacterial and Virus Strains
Encephalomyocarditis virus (EMCV)-K Gorman et al, Nat Immunol. 2017 Jul; 18(7): 744–752 N/A
Chemicals, Peptides, and Recombinant Proteins
2BAct mouse chow Wong et al., eLife 2019; 8:e42940 N/A
Envigo Teklad Global 14% Protein Rodent Maintenance Diet Envigo Cat # 2914
BioServ Rodent Reproductive Diet Bio Serv Product# S3823P
Recombinant mouse Interferon beta protein R&D Systems Cat # 12405-1
Recombinant human interferon beta protein R&D Systems Cat # 11415-1
ISRIB SIGMA Cat # SML0843
Critical Commercial Assays
Taq Man Gene Expression Assays Thermo Fisher Cat # 4351372
Deposited Data
RNA Seq raw data of P195A/p150+ and P195A/p150− liver, kidney, and cerebellum GEO GSE162583
Experimental Models: Cell Lines
C57BL/6 primary MEFs, (mouse embryonic fibroblasts) Gray EE et al, J Immunol 2015 195:1939 N/A
HEK 293T cells ATCC CRL-3216
A549 cells ATCC CCL-185
mouse bone marrow macrophages, C57BL/6J Gray EE et al, J Immunol 2015 195:1939 N/A
Experimental Models: Organisms/Strains
mouse: Adar P195A: C57BL/6J this paper N/A
mouse: Eif2ak2−/−: C57BL/6J-Eif2ak2tm1Jcbe Nakamura et al. Cell Rep. 2015 Apr 14; 11(2): 295–307. N/A
mouse: Adar p150+/−: C57BL/6J Pestal et al. Immunity 2015 Nov 17;43(5):933–44. N/A
mouse: Adar+/−: C57BL/6J Pestal et al. Immunity 2015 Nov 17;43(5):933–44. N/A
mouse: Dhx58−/−: C57BL/6J-Dhx58tm1Itl Suthar et al. Immunity 2012 Aug 24; 37(2): 235–248. N/A
mouse: Ifih1−/−: C57BL/6J-Ifih1tm1.1Cln Gitlin et al. Proc Natl Acad Sci 2006 May 30;103(22):8459–64. N/A
mouse: RnaseI−/−: C57BL/6J-RnaseItm1Slvm Zhou et al. EMBO. 1997 Nov 3;16(21):6355–63. N/A
mouse: Ifnar1−/−: C57BL/6J-Ifnar1tm1Agt Kolumam et al. J Exp Med. 2005 Sep 5;202(5):637–50. N/A
Oligonucleotides
See Table S1 for qPCR primer sequences See table S1 N/A
See Table S2 for guide RNA sequences See table S2 N/A
Recombinant DNA
pRRL lentiCRISPR vectors. Guide RNA sequences in Table S2 Gray et al. Immunity. 2016 Aug 16;45(2):255–66 N/A
Software and Algorithms
Prism v 9.0 GraphPad N/A
Adobe Photoshop Elements 2020 Adobe N/A
Galaxy FASTQ Trimmer tool v1.0.0 N/A N/A
FastqMcf (v1.1.2) N/A N/A
STAR aligner (v2.4.2a) N/A N/A
HTSeq-count (v0.4.1) N/A N/A
PICARD (v1.134) N/A N/A
FASTQC (v0.11.3) N/A N/A
Samtools (v1.2) N/A N/A
HTSeq-count (v0.4.1) N/A N/A
DESeq2 Love et al., Genome Biol 2014 15,550 N/A
fgsea Koretkevich et al., 2019 https://github.com/ctlab/fgsea/