Figure 1.

Single-component optogenetic control over RhoA signaling. a. Schematized induction of cytoskeletal changes and contractile signaling in response to opto-RhoA activation by dynamic membrane recruitment. b. Epifluorescence micrographs of HEK cells expressing opto-RhoA, visualized by mCherry. i. Trailing edge contraction in two adjacent cells. ii. Four-cell adherens junction separation. White box = spatially patterned blue light illumination field, stimulated at 1.6% duty ratio. Dotted yellow line = cell boundary mask in the dark-adapted state. Scale = 10 μm.