Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Sep 3;9:735971. doi: 10.3389/fcell.2021.735971

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Chen, Fan, Liu, Chen, Xie, Zeng, Wen, Li, Ma, Ou, Luo and Guo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Making holding and microinjection pipettes. (A) The capillary glass tube, the micropipette puller, and the microforging were used to make the holding and microinjection needles in this study. (B) Three types of the needle are produced from the micropipette puller. The needles with a symmetrical tail and small tip are ideal to make microinject pipettes. (C) The cutting diameter should be about 50 μm, which is good for making a holding pipette (the objective lens is ×10). At a position about 2.5 mm away from the end of the pipette, the holding and microinjection needle will be bent for 15–20° (the objective lens is ×5).