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. 2021 Sep 3;12:682155. doi: 10.3389/fmicb.2021.682155

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Copyright © 2021 Tsushima, Narusaka, Gan, Kumakura, Hiroyama, Kato, Takahashi, Takano, Narusaka and Shirasu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Transient expression of GFP-tagged ChCEC3 protein-induced nuclear expansion in N. benthamiana leaf cells. In merged images, green represents GFP signals, cyan represents DAPI signals, and magenta represents chlorophyll autofluorescence. Open arrowheads indicate expanded nuclei. Images were taken 24 h after infiltration. Bars = 10 μm. (B) Boxplots of nuclear diameters resulting from transient expression of GFP-tagged CEC3 proteins. Data represent the medians of biological replicates. N represents the number of nuclei examined. CoCEC3-2.2ΔSP-GFP is not included because no GFP signal was detected. Analysis of variance with Tukey post hoc honestly significant difference test (P < 0.05) was performed.