FIGURE 2.

Phagocytosis of pHrodo non‐typeable Haemophilus influenzae (NTHi) in CD66b⁺ and CD206⁺ sputum cells. Sputum cells from 14 chronic obstructive pulmonary disease (COPD) patients, 8 healthy smokers (HS) and 9 healthy never‐smokers (HNS) were either unexposed or exposed with pHrodo NTHi bacteria for 30 min or 3 h. Cells were then harvested and stained with CD45, CD66b and CD206 and the data acquired using the BD Canto II flow cytometer. CD45⁺CD206⁺ and CD45⁺CD66b⁺ cells were gated and the percentage of NTHi‐positive cells (a) was analysed after 30 min (b) and 3 h (c) by FlowJo. Data are representative from HNS (a) and presented as mean ± standard error of the mean (SEM) (b,c). Statistical significance is shown above corresponding bars [ordinary analysis of variance (ANOVA) followed by Tukey’s multiple comparison test between groups for each cell type, paired t‐tests between cell types within each group]. Significance between CD206⁺ and CD66b⁺ cells (*p < 0.05, **p < 0.01 and ***p < 0.001, respectively). Significance between groups for CD206⁺ cells (^# p < 0.05)