FIGURE 3.

The effects of pHrodo non‐typeable Haemophilus influenzae (NTHi) exposure on CD206^+/− CD14^high/low expression by sputum cells. Sputum cells from 14 chronic obstructive pulmonary disease (COPD) patients, 8 healthy smokers (HS) and 9 healthy never‐smokers (HNS) were either unexposed or exposed with pHrodo NTHi bacteria for 3 h. Cells were then harvested and stained with CD45, CD66b, CD206 and CD14 and the data acquired using the BD Canto II flow cytometer. CD45⁺CD66b⁻ cells were gated and the percentage of CD206⁺CD14^high (a), CD206⁺CD14^low (b), CD206⁻CD14^high (c) and CD206⁻CD14^low (d) populations analysed by FlowJo. Data are presented as mean ± standard error of the mean (SEM). Statistical significance is shown above corresponding bars (t‐test). Significance between unexposed and NTHi exposed (*p < 0.05, **p < 0.01 and ***p < 0.001, respectively)