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. 2021 Sep 3;12:671487. doi: 10.3389/fpls.2021.671487

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Copyright © 2021 Koudounas, Thomopoulou, Rigakou, Angeli, Melliou, Magiatis and Hatzopoulos.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In vitro enzymatic assays of crude leaf protein extracts of olives Agroinoculated with either pTRV2-EV or pTRV2-OeGLU constructs. (A) Relative enzymatic activity against oleuropein at 6 min. (B) Relative enzymatic activity against oleuropein at regular time intervals. (C) Relative enzymatic activity against pNPGlu. Three technical replicates of three biological replicates per treatment were analyzed. Mean ± SE and asterisks denote statistical significance (***P ≤ 0.001, **P ≤ 0.01, *P ≤ 0.05, ns: not significant; Student’s t-test). EV, empty vector; OeGLU, oleuropein β-glucosidase; pNPGlu, p-nitrophenyl β-D-glucopyranoside.