FIGURE 4.
Signal-to-noise ratio versus detection area. Representative optical recording of optogenetically induced APs collected from different-sized surface areas (2 Hz stimulation frequency with blue light pulses duration of 30 ms). Reduction of the detection area from the whole well area (≈190 mm2) to 0.17 mm2, reduced the signal-to-noise ratio from ≈ 600 to ≈ 2. The estimated number of cells present in the different-sized detected area is indicated in the right column.