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. 2021 Sep 3;12:692496. doi: 10.3389/fphys.2021.692496

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Copyright © 2021 Credi, Balducci, Munagala, Cianca, Bigiarini, de Vries, Loew, Pavone, Cerbai, Sartiani and Sacconi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Patch-clamp recording of AP in HL-1 cells. (A) Representative AP recording on a HL-1 cells using single cell patch-clamp (whole-cell configuration). APs were elicited at 0.2 Hz in current-clamp mode. (B) Table reporting average ± standard error of maximal diastolic potential (MDP), APA, APD₅₀, and APD₉₀ measured in patch-clamp cells (number of cells = 15). APA, APD₅₀, and APD₉₀ measured with MULTIPLE (pulses duration of 30, 40, and 50 ms) at the same stimulation frequency (0.2 Hz) are reported for direct comparison.