Figure 6.

The impact of ARCL1C causing point mutation on LTBP4 N-terminal interaction with fibulin-4. Binding studies using BLI were performed using OctetRED analysis. Representative Octet sensorgrams showing both (A) (i) NWT and (B) (i) C274G at a range of concentrations (1000–62.5 nm) bind to immobilised fibulin-4. The binding affinity KD was determined by steady state (equilibrium) for (A) (ii) the wild type and (B) (ii) C274G. All experiments were performed in at least duplicate and representative results are shown.