Table 1.
Experimental hydrodynamic results for LTBP4 C-terminal constructs.
| Hydrodynamic properties | NWT | C274G | CWT | C1286S | C1186R |
|---|---|---|---|---|---|
| Molecular mass (kDa)a | 47 | 51 | 58 | 58 | 58 |
| Hydrodynamic radius Rh (nm)a | ND | ND | 4.7 | 4.7 | 5.0 |
| Sedimentation coefficient (Sw)b | 3.15 | 3.54 | 4.12 | 4.24 | 3.82 |
| Sedimentation coefficient (Sw,20)b | 3.25 | 3.61 | 4.05 | 4.16 | 3.75 |
| Frictional ratiob | 1.40 | 1.33 | 1.39 | 1.31 | 1.49 |
| Rh (nm)b | 3.20 | 3.14 | 3.70 | 3.61 | 3.83 |
| Radius of gyration Rg (nm)c | 3.6 | 3.5 | 3.9 | 4.0 | 4.2 |
| Maximum dimension Dmax (nm)c | 18.0 | 15.7 | 18.0 | 18.0 | 18.7 |
a
Multi-Angle Light Scattering (MALS).
b
Sedimentation Velocity Analytical Ultracentrifugation (SV-AUC).
c
Small-Angle X-ray Scattering (SAXS).