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. 2021 Sep 3;12:701529. doi: 10.3389/fpls.2021.701529

Figure 8.

Figure 8

Subcellular localization and transcriptional regulation activity of OsS40. (A) Subcellular co-localization of OsS40s-GFP and OsWRKY44-RFP proteins in rice protoplasts as revealed by green fluorescence and red fluorescence, respectively. Scale bar = 5 μm (B) A scheme of reporter and effector constructs used in the transcriptional regulation assay. The GAL4-responsive reporter constructs, GAL4-LUC, containing five copies of the GAL4-binding site in tandem and a minimal TATA region (starting at position 46) of the CaMV 35S promoter, the firefly gene for luciferase (LUC), and a nopaline synthase (nos) terminator. Each effector construct contained a GAL4 DNA-binding domain (GAL4DB) and a coding region of OsS40 driven by the CaMV 35S promoter. (C) Effects of the OsS40 on reporter gene expression as revealed by relative LUC activity. The GAL4 DNA-binding domain (DB) and VP16 were used as negative and positive controls, respectively. Mean and SD values were obtained from three biological replicates. Significant differences of the LUC levels normalized to GAL4 DB were evaluated using Student's t-test (*p < 0.05; **p < 0.01, ***p < 0.001).