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. 2021 Sep 3;13:722580. doi: 10.3389/fnagi.2021.722580

FIGURE 1.

FIGURE 1

(A) Experimental design and the test order. Aβ 42 was microinfused into bilateral prefrontal cortices of mice on day 4 after surgery. Lentiviral vectors containing negative control sequence (NC) or 4DmiRNA were microinfused into bilateral prefrontal cortices of mice on day 5 after surgery. H89 or artificial cerebrospinal fluid were microinjected once daily starting from days 8 to 22. Behavioral experiments were performed from days 19 to 22, 30 min after H89 treatment. Animals were sacrificed for biochemical assays after behavioral tests (day 23). OF, open field test; NOR, novel object recognition test; PA, passive avoidance test; 8-RAM, eight-arm radial maze; SAC, sacrifice. (B) Photomicrographs of representative cannula placements in the brain region of microinjection are indicated by the red ellipse corresponding to the region. (C) PDE4 subtypes expression in the prefrontal cortex. PDE4D expression, but not PDE4A and PDE4B, was decreased after microinjection of lenti-PDE4D-miRNA into the prefrontal cortex for 2 weeks. Values were the means ± SEM with 12 mice in each group. ***P < 0.001 vs. vehicle-treated control group (NC).