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. 2021 Sep 3;12:726878. doi: 10.3389/fmicb.2021.726878

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Copyright © 2021 Zhuang, Huang, Liu, Liu, Tu, Yin and He.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of RSV on the levels of intestinal barrier proteins in weaned mice. (A) The relative mRNA expression of claudin-1, occludin, and ZO-1 was detected by qRT-PCR. (B) The protein levels of Claudin-1, Occludin and ZO-1 were detected by Western blot with β-actin as the loading control. Con = control group, RSV10 = 10 mg/kg RSV, RSV20 = 20 mg/kg RSV, RSV50 = 50 mg/kg RSV. *Means with single asterisks are significantly different (p < 0.05) from values of control group, and all the data are expressed as the means ± SEM.