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. 2021 Jul 26;10(18):6416–6427. doi: 10.1002/cam4.4159

FIGURE 4.

FIGURE 4

DYRK1A decreased NFATC2 protein expression and transcriptional activity. A, DYRK1A decreased protein expression of NFATC2 in T98G cells. T98G cells were transfected with pCMV‐DYRK1A. NFATC2 and DYRK1A were detected using NFATC2 monoclonal antibody and DYRK1A polyclonal antibody, respectively. ACTB was used as the loading control. Values represent means ±SD, n = 3. p < 0.05 by student t‐test. B, The mRNA levels of NFATC1 target genes IL2 and TNFα were decreased by DYRK1A overexpression. pWT‐NFATC2 was co‐transfected with pCMV‐DYRK1A into T98G cells. qRT‐PCR was used to detect NFATC2‐targeted gene expression of IL2 and TNFα. C, NFATC2 transcriptional activity was decreased by DYRK1A. T98G cells were co‐transfected with NFATC2 activity reporter pNFATluc, p‐NFATC2mycflag, and pCMV‐DYRK1A. Dual luciferase assay was performed 48 h after transfection. Values represent means ±SD, n = 3. p < 0.05 by student t‐test