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. 2021 Sep 3;11:688882. doi: 10.3389/fonc.2021.688882

Figure 3.

Figure 3

MiR-1236-3p inhibited colon cancer invasion and migration in vitro. Transwell migration (A) and invasion (B) assays in SW620 and Caco-2 cells transfected with miR-1236-3p mimic or mimic control. Scale bar: 100μm (C) Wound-healing assay at 0, 24, and 48 h in SW620 and Caco-2 cells transfected with miR-1236-3p mimic or mimic control. (D) Western blotting analysis of E-cadherin, N-cadherin, and vimentin in SW620 cells transfected with miR-1236-3p mimic or mimic control. (E) Western blotting analysis of MMP-2, MMP-9, and CXCR-4 in SW620 cells transfected with miR-1236-3p mimic or mimic control. (F) Morphologic changes in SW620 cells transfected with miR-1236-3p mimic or mimic control by inverted microscope. Scale bar: 100μm. MMP-2, matrix metalloproteinase-2; MMP-9,matrix metalloproteinase-9; CXCR-4, C-X-C Motif Chemokine Receptor 4. Error bars represent the mean ± s.e.m. ***P < 0.001 by Student’s t-test, six replicates per group for each experiment.