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. 2021 Sep 3;11:688882. doi: 10.3389/fonc.2021.688882

Figure 6.

Figure 6

Identification of DCLK3 as the target gene of miR-1236-3p. (A) Identification of DCLK3 as the target gene by vein result using upregulated genes and four online target-predicting algorithms (TargetScan, miRDB, miRWalk, and miRTarBase). (B) The binding sites of DCLK3 and hsa-miR-1236-3p by TargetScan. (C) Luciferase reporter activity after transfections (mimic control, miR-1236-3p mimic, inhibitor control, and miR-1236-3p inhibitor) in SW620 and Caco-2 cell lines. The results of qRT-PCR (D) and western blotting (E) indicated that miR-1236-3p mimic decreased the DCLK3 expression, while miR-1236-3p inhibitor increased the DCLK3 expression in both SW620 and Caco-2 cell lines. qRT-PCR, quantitative reverse transcription–polymerase chain reaction; Error bars represent the mean ± s.e.m. **P < 0.01, ***P < 0.001 by Student’s t-test, six replicates per group.