Table 2.
Sequences of miR-1236-3p mimic, inhibitor, siRNAs, and primers for qRT-PCR.
| Name | Sequence (5′-3′) |
|---|---|
| DCLK3 | |
| Forward | CCAGCAGAAATTCAAGCATATGTCC |
| Reverse | ACAATCTGTTTATCCATGCACCA |
| GAPDH | |
| Forward | GACCTGACCTGCCGTCTAG |
| Reverse | AGGAGTGGGTGTCGCTGT |
| U6 | |
| Forward | GAGAAGATTAGCATGGCCCCT |
| Reverse | ATTTCTCGATTTGTGCGTGTCA |
| miR-1236-3p | |
| RT-primer | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCTGGAG |
| Forward | AACAAGCCTCTTCCCCTTGT |
| Reverse | GTCGTATCCAGTGCAGGGT |
| miR-1236-3p mimic | |
| Forward | CCTCTTCCCCTTGTCTCTCCAG |
| Reverse | GGAGAAGGGGAACAGAGAGGTC |
| miR-1236-3p inhibitor | CCTCTTCCCCTTGTCTCTCCAG |
| Si-DCLK3 | |
| Forward | UAAAAAGGGCCAGUUUUGCUG |
| Reverse | GCAAAACUGGCCCUUUUUAUC |
qRT-PCR, quantitative reverse transcription–polymerase chain reaction.