FIGURE 4.

Photomicrographs illustrating Iba1‐ir microglia in the corpus callosum after laparotomy (A) or cecal ligature and puncture (B) in rats that did not undergo MRI under isoflurane anesthesia. (For illustrations of Iba‐1‐immunoreactivity in animals that underwent MRI under isoflurane anesthesia, see figure 8.¹⁵) Quantitative Fraclac‐based analysis of microglial circularity (C) and width (D) in the corpus callosum showing means ± SEM for the four experimental groups. ir: immunoreactivity *P < .05. Group sizes Sham MRI−: n = 4; CLP MRI−: n = 4; Sham MRI+: n = 9; CLP MRI+: n = 11. Scale bar = 100 μm