TABLE 1.
Effects of CLP and MRI under anesthesia on brain perivascular IgG diffusion
| Region | Occurrence × extent/section | ANOVA & Post hoc (Benjamini–Hochberg corrected) |
|---|---|---|
| SSp |
Sh.+: 0.033 ± 0.022; Sh.−: 0.333 ± 0.183 CLP+: 0.061 ± 0.019; CLP−: 0.137 ± 0.066 |
MRI P < .01 |
| SSs |
Sh.+: 0.023 ± 0.023; Sh.−: 0.176 ± 0.054 CLP+: 0.027 ± 0.018; CLP−: 0 |
Surgery P < .01; Surgery × MRI P < .01 Sh.+ < Sh.− P < .001 Sh.− > CLP− P < .001 |
| VISC |
Sh.+: 0; Sh.−: 0.085 ± 0.043 CLP+: 0.004 ± 0.004; CLP−: 0.019 ± 0.019 |
MRI P <.01; Surgery × MRI P < .05 Sh.+ < Sh.− P < .01 Sh.− > CLP− P < .01 |
| GU |
Sh.+: 0.017 ± 0.017; Sh.−: 0.039 ± 0.023 CLP+: 0.003 ± 0.003; CLP−: 0 |
Surgery P < .05 |
| AI |
Sh.+: 0; Sh.−: 0.057 ± 0.037 CLP+: 0.011 ± 0.011; CLP−: 0.008 ± 0.008 |
NS |
| PIR |
Sh.+: 0.026 ± 0.011; Sh.−: 0.106 ± 0.077 CLP+: 0.020 ± 0.009; CLP−: 0.016 ± 0.012 |
NS |
| TT |
Sh.+: 0.010 ± 0.010; Sh.−: 0.038 ± 0.038 CLP+: 0; CLP−: 0 |
NS |
| CP |
Sh.+: 0.048 ± 0.017; Sh.−: 0.277 ± 0.157 CLP+: 0.119 ± 0.042; CLP−: 0.028 ± 0.028 |
NS |
| GPe |
Sh.+: 0; Sh.−: 0.019 ± 0.019 CLP+: 0; CLP−: 0 |
NS |
| DG |
Sh.+: 0.018 ± 0.018; Sh.−: 0.028 ± 0.028 CLP+: 0; CLP−: 0 |
NS |
| cc‐ec |
Sh.+: 0.101 ± 0.079; Sh.−: 0.471 ± 0.234 CLP+: 0.142 ± 0.069; CLP−: 0.384 ± 0.135 |
NS |
| ec |
Sh.+: 0.026 ± 0.015; Sh.−: 0.147 ± 0.086 CLP+: 0.063 ± 0.038; CLP−: 0.141 ± 0.057 |
NS |
| fi |
Sh.+: 0.116 ± 0.078; Sh.−: 0.059 ± 0.028 CLP+: 0; CLP−: 0.015 ± 0.015 |
NS |
Occurrence multiplied by extension score of perivascular of IgG staining per section for different brain structures. The left column displays brain structures based on Swanson's rat brain atlas,74 the middle column shows means ± SEM for the four experimental groups and the right column contains a summary of ANOVA and post hoc tests (when appropriate).
Abbreviations: AI, agranular insular cortex; cc‐ec, corpus callosum‐external capsule transition zone; CLP−, Cecal ligature and puncture‐operated animals that did not undergo MRI under isoflurane anesthesia 24 h later; CLP+, Cecal ligature and puncture‐operated rats that were subject to MRI under isoflurane anesthesia one day later; CP, caudate putamen; DG, dentate gyrus; ec, external capsule; fi, fimbria; GPe, external globus pallidus; GU, gustatory area; NS, nonsignificant; PIR, piriform cortex; Sh−, Sham‐operated animals that did not undergo MRI under isoflurane anesthesia 24 h later; Sh+, Sham‐operated rats that were subject to MRI under isoflurane anesthesia one day later; SSp, primary somatosensory cortex; SSs, secondary somatosensory cortex; TT, tenia tecta; VISC, visceral area.