Fig. 2.

ATM and RGS6 are up-regulated in NAFLD. (A) Histological characterization of NAFLD human liver samples. Detectable disruption of liver architecture (H & E), fibrosis (Masson Trichrome), inflammation (F4/80), regeneration (PCNA) and cell death (TUNEL) [scale bar = 100 μm] was observed in livers from NAFLD patients. F4/80, PCNA, and TUNEL positive cells are quantified (n = 10). (B) Immunohistochemical staining for RGS6 and ATM in liver of control and NAFLD patient liver samples (n = 10). Representative images and quantification across samples are shown. (C) NAFLD samples were stratified based on RGS6 expression (low, n = 7; medium, n-10; high, n = 9) and immunoblotting performed for RGS6, ATM, and γH2AX. (D) Densitometric quantification was performed, and protein content expressed relative to RGS6 low samples. β-Actin is used as a loading control for all immunoblots.(E) Correlation between ATM and RGS6 expression across 48 liver samples detected via immunoblotting. A simple linear regression was used to determine the goodness of fit (r²) and degree to which the slope of the line deviates from zero (P). Data were analyzed by student's t-test or one-way ANOVA with Sidak's post-hoc test. *P < 0.05, ***P < 0.001, ****P < 0.0001. ns = not significant. Data are presented as mean ± SEM.