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. 2021 Aug 18;297(3):101094. doi: 10.1016/j.jbc.2021.101094

Figure 2.

Figure 2

Proteomic analysis of major C. elegans tissues.A, western blot of lysates obtained from animals expressing free TurboID specifically in the neurons, muscle, intestine, and hypodermis. B, total, tissue-enriched, and tissue-specific proteins detected in various tissues by mass spectrometry. Tissue enrichment is defined as proteins with 2-fold or greater mean spectral counts compared with the second-ranked sample. Tissue-specific proteins were uniquely identified in the corresponding tissue. C, Venn diagram showing the distribution of protein hits between samples. D, mean spectral count of representative proteins in various tissues obtained via mass spectrometry. E and F, mean spectral counts of the proteins Y53G8AL.1, B0379.1, T01G5.1, and R10E8.8 encoded by genes predicted by our TurboID experiments (E), and confocal microscopy images of worms expressing mNeongreen from the promoters of these genes (F). Scale bars: 100 μm.