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. 2021 Aug 18;297(3):101094. doi: 10.1016/j.jbc.2021.101094

Figure 3.

Figure 3

Proteomic analysis of the AFD sensory neuron pair.A, confocal microscopy image of C. elegans expressing TurboID::mNeongreen fusion protein specifically in the AFD neurons. B, western blot analysis of worm lysates obtained from samples expressing free TurboID in AFD neurons, all neurons or none (WT). C, Venn diagram showing the distribution of protein hits between samples. D, the AFD markers GCY-8, TTX-1, and GCY-18 are highly enriched in AFD::TurboID samples. E, NEX-4, F37A4.6, and T06G6.3 proteins were highly enriched in AFD::TurboID samples. F, Confocal microscopy images of C. elegans expressing nex-4p::mNeongreen, F37A4.6p::mNeongreen and T06G6.3p::mNeongreen transgenes. A gcy-8p::mKate transgene was used as a fiducial marker to identify AFD neurons. A and F, scale bars: 50 μm.