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. 2021 Aug 18;297(3):101094. doi: 10.1016/j.jbc.2021.101094

Figure 4.

Figure 4

Interactome analysis of the presynaptic active zone protein ELKS-1.A, confocal microscopy image of C. elegans harboring an elks-1::TurboID::mNeongreen knock-in allele generated by CRISPR/Cas9-mediated genome editing. The nerve ring, where many synapses are located, is marked with a white arrow. B, western blot analysis of lysates obtained from animals expressing the ELKS-1::TurboID::mNeongreen fusion protein. C, Venn diagram showing the distribution of protein hits between samples. D, the synaptic proteins ELKS-1/ELKS, UNC-10/RIM, SYD-1/SYDE1, SYD-2/liprin-alpha, SAD-1/BRSK1, CLA-1/CLArinet, C16E9.2/Sentryn, and RIM binding protein RIMB-1 were highly enriched in ELKS-1::TurboID samples. E, C03H5.6, C11E4.6 and H06I04.1 were highly enriched in ELKS-1::TurboID samples. F, confocal microscopy images of transgenic C. elegans expressing C03H5.6::mNeongreen, C11E4.6::mNeongreen and H06I04.1::mNeongreen translational fusions in the AFD neuron pair. elks-1::mScarlet, used as a control to mark AFD synapses, confirms these proteins are synaptic. Scale bars: 10 μm (A) and 5 μm (F).