Table 1.
List of plasmids used for double transformations (pACYC vector with/without protease, pETDuet vector with cp ATCase) into E. colipyr− cells and ability to overcome pyrimidine auxotrophy
| Substrate plasmid MCSI/MCSII of pETDuet-1 | Protease plasmid |
|
|---|---|---|
| Empty pACYC (Mock) | pACYC cp caspase-2 | |
| pyrI/cp pyrB | a | a |
| pyrI/6H-VDVAD-cp pyrB | b | a |
| pyrI/6H-WEHD-cp pyrB | b | b |
a
Cells able to form distinct colonies on selective agar within 24 to 48 h.
b
Cells not able to form colonies on selective agar within 24 to 48 h.