. 2021 Aug 19;297(4):101095. doi: 10.1016/j.jbc.2021.101095

Table 2.

List of plasmids used for double transformations into E. colipyr− cells (pACYC vector with/without protease, pETDuet vector with ATCase) and ability to overcome pyrimidine auxotrophy

Substrate plasmid MCSI/MCSII of pETDuet-1	Protease plasmid
Substrate plasmid MCSI/MCSII of pETDuet-1	Empty pACYC (Mock)	pACYC M^pro
pyrI/cp pyrB	^a	^a
pyrI/cp pyrB T228S	^a	n. d.
pyrI/6H-AVLQ-S-cp pyrB	^b	^a

Cells able to form distinct colonies on selective agar within 24 to 48 h.

Cells not able to form colonies on selective agar within 24 to 48 h, n. d. not determined.