. 2021 Jul 13;32(9):1999–2013. doi: 10.1021/acs.bioconjchem.1c00292

Table 2. Membrane Affinity of PAs Studied by Flow Cytometry and Trp Fluorescence Emission.

		flow cytometry assay	binding assay
hydrophobic moiety	PA	% membrane associated-fluorescence	K_x^a
monoalkyl	N-PA_monoalkyl	36	1.2 × 10^–6
	C-PA_monoalkyl	63	3.8 × 10^–6
	K-PA_monoalkyl	41	0.24 × 10^–6
dialkyl	N-PA_dialkyl	25	ND
	C-PA_dialkyl	27	ND
	K-PA_dialkyl	26	ND
chol	N-PA_chol	32	ND
	C-PA_chol	47	2.4 × 10^–6
	K-PA_chol	51	0.9 × 10^–6

K_x values of apparent mole fraction partition coefficients determined from partitioning isotherms of PAs estimated from the fractional change in Trp fluorescence intensity upon addition of increasing amounts of liposomes.