Figure 6. Proteinuric kidney injury stimulates ileal macrophage production of VEGF-C.
(A) PAN increased ileal VEGF-C vs controls. (B) VEGF-C concentration in PAN lymph was lower but total output of VEGF-C was significantly greater in PAN vs controls. (C) Double staining of ileum with VEGF-C (red) and CD68 (green) showed greater number of CD68-positive cells and CD68 & VEGF-C-positive cells (arrows) in PAN vs controls. (D) Cultured macrophages exposed to IsoLG-apoAI expressed more VEGFC mRNA vs unmodified apoAI. In vivo, results are mean ± SD for 7–12 rats per group. In vitro, experiments were performed independently 3 times with 3 wells per treatment.