Fig. 3.

Effects of increasing the dose of Sigma poly I:C. a) C57BL/6J female adult mice were placed in an open field 2 h 50 m post-treatment and measures of locomotion (lower beam breaks) and rears (upper beam breaks) were accumulated over a 10 m trial period. b) Systemic expression of the pro-inflammatory cytokines IL-6, IFNβ and TNFα were determined using ELISA. c) Transcription of the pro-inflammatory cytokines Il6, Ifnb and Tnfa in liver tissue were determined using real time PCR. Data represent mean +/- SEM for saline (n = 4), 20 mg/kg Amersham poly I:C (n = 4), 40 mg/kg Sigma poly I:C (n = 5) and 80 mg/kg Sigma poly I:C (n = 5). All data were compared using one-way ANOVA, followed by Bonferroni post-hoc tests comparing differences between each treatment group. *p < 0.05, **p < 0.01 and ***p < 0.001 denote significant differences between saline and the represented poly I:C preparation. ^$p < 0.05, ^$$p < 0.01 and ^$$$p < 0.001 denote significant differences between 40 mg/kg Sigma poly I:C and the represented poly I:C preparation. ⁺p < 0.05, ⁺⁺p < 0.01 and ⁺⁺⁺p < 0.001 denote significant differences between 20 mg/kg Amersham poly I:C and represented poly I:C preparation.