Fig. 6.

Impact of age on innate immune responses to poly I:C. a) Pro-inflammatory cytokines in plasma were measured by ELISA (IL- 1β, TNF-α, IL-6 and IFNβ). Expression of cytokines in the hypothalamus (b) and hippocampus (c) was determined using quantitative PCR. All data were compared using two-way ANOVA followed by Bonferroni post-hoc tests. Poly I:C had a significant main effect on all cytokines in all areas. Significant interactions of treatment with age are indicated by *p < 0.05, **p < 0.01, ***p < 0.001 and significant post-hoc differences between poly I:C in young and aged by # p < 0.05 (n = 6 for all groups except young + saline; n = 5). All data are represented by mean+/- SEM.