Table 2.
Gene | Oligo | Sequence | Amplicon (bp) |
---|---|---|---|
Irf7 | Forward Reverse |
5′ - CGAGGAACCCTATGCAGCAT − 3′ 5′ – TACATGATGGTCACATCCAGGAA − 3′ |
180 |
Cxcl10 | Forward Reverse Probe |
5′ - GCCGTCATTTTCTGCCTCAT − 3′ 5′ - GCTTCCCTATGGCCCTCATT − 3′ 5′ – TCTCGCAAGGACGGTCCGCTG − 3′ |
127 |
Isg15 | Forward Reverse Probe |
5′ - CGCACTGTAGACACGCTTA − 3′ 5′ - CCCTCGAAGCTCAGCCAG − 3′ 5′ – 6TCCAGCGGAACAAGTCACGA − 3′ |
79 |
SYBR green (Applied Biosystems, Roche, UK) used in place of probe, where probe is not stated.