Table 5.
Fatty acid composition in S. elongatus expressing the empty vector pSyn6, CzFAD3A or CzFAD3B
| Fatty acids (%) | Without FFA feeding | With C18:2Δ9,12 feeding | With C18:3Δ9,12,15 feeding | ||||||
|---|---|---|---|---|---|---|---|---|---|
| pSyn6 | CzFAD3A | CzFAD3B | pSyn6 | CzFAD3A | CzFAD3B | pSyn6 | CzFAD3A | CzFAD3B | |
| C16:0 | 40.0 ± 3.2 | 38.1 ± 2.7 | 36.7 ± 2.2 | 39.9 ± 2.3 | 31.7 ± 1.9 | 38.9 ± 4.0 | 38.9 ± 3.6 | 30.7 ± 1.1 | 46.1 ± 1.5 |
| C16:1Δ4 | – | 12.7 ± 1.1 | – | – | 8.7 ± 1.2 | – | – | 12.3 ± 1.1 | – |
| C16:1Δ9 | 45.1 ± 5.1 | 40.6 ± 3.1 | 43.8 ± 1.9 | 42.7 ± 5.0 | 39.1 ± 1.4 | 37.1 ± 2.7 | 35.1 ± 1.4 | 35.0 ± 2.0 | 34.8 ± 3.2 |
| C18:0 | 1.1 ± 0.2 | 1.6 ± 0.1 | 2.8 ± 0.2 | 1.1 ± 0.2 | 2.1 ± 0.3 | 3.7 ± 0.2 | 1.2 ± 0.1 | 1.4 ± 0.6 | 1.5 ± 0.1 |
| C18:1Δ9 | 4.1 ± 0.9 | 2.2 ± 0.3 | 5.2 ± 0.9 | 3.1 ± 0.5 | 4.3 ± 0.7 | 4.2 ± 0.1 | 2.4 ± 0.3 | 2.2 ± 0.2 | 1.7 ± 0.1 |
| C18:1Δ11 | 8.7 ± 1.8 | 4.8 ± 0.7 | 11.6 ± 1.0 | 6.7 ± 0.7 | 7.9 ± 0.6 | 9.3 ± 1.6 | 7.1 ± 0.9 | 5.0 ± 1.4 | 4.7 ± 0.3 |
| C18:2Δ9,12 | – | – | – | 6.6 ± 0.2 | 6.2 ± 1.4 | 3.5 ± 0.3 | – | – | – |
| C18:3Δ6,9,12 | – | – | – | – | – | 3.4 ± 0.6 | – | – | – |
| C18:3Δ9,12,15 | – | – | – | – | – | – | 15.4 ± 1.1 | 12.6 ± 1.3 | 2.9 ± 0.2 |
| C18:4Δ6,9,12,15 | – | – | – | – | – | – | – | 0.9 ± 0.0 | 8.3 ± 0.7 |