Figure EV1. Analysis of SARS‐CoV‐2 protein expression by Western blotting and interferon (IFN) signaling assays (related to Fig 1).

• A
  Twenty‐nine SARS‐CoV‐2 genes with the SFB tag were expressed in cells. Cells transfected with vector or construct encoding SFB‐tagged GFP were included as controls. Western blotting was conducted with indicated antibodies. Red arrows indicate the predicted positions of bait gene products.
• B
  Twenty‐nine SARS‐CoV‐2 genes with the second‐generation biotin ligase (BioID2) tag were expressed in cells. Untransfected cells, cells transfected with vector, and cells transfected with construct encoding GFP tagged with BioID2 were included as controls. Western blotting was conducted with indicated antibodies. Red arrows indicate the predicted positions of bait gene products.
• C
  Validation of BioID2 labeling by blotting with streptavidin antibody.
• D
  Analysis of IFN‐beta‐luciferase reporter 12 h after Sendai virus (SeV) infection with co‐expression of indicated SARS‐CoV‐2 viral proteins.
• E
  Analysis of ISRE‐luciferase reporter when the indicated SARS‐CoV‐2 viral proteins were co‐expressed with IRF3.

Data information: Graphs D and E show mean ± SD, n = 3. **P < 0.01 and *P < 0.05 (Student’s t‐test).Source data are available online for this figure.