Figure 1. Phylogenetic analysis of HOs.

1. A phylogenetic tree of 161 different HO sequences was calculated. HOs were aligned with Clustal Omega. Afterward, the sequences were trimmed by trimAL with gap threshold 0.8 and conservation percentage of 70%. The tree was calculated with PhyML with 200 bootstrap cycles and the AIK parameter switched on. Visualization was done in iTOL. Within the two fungal clades, two lichen‐derived sequences are shaded in dark blue.
2. Alignment of the heme‐binding region of several HOs. The heme‐coordinating histidine is indicated with a star. HoxB lacks this histidine, like HO‐2 from A. thaliana.