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. 2021 Jul 13;40(17):e108083. doi: 10.15252/embj.2021108083

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© 2021 The Authors. Published under the terms of the CC BY NC ND 4.0 license

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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A, B
Biolayer interferometry analysis with Apo‐PGP A and holo‐PGP B. Proteins were immobilized at a streptavidin sensor tip. Free binding sites were blocked with biotin. Association and dissociated kinetics were recorded at the indicated HoxA concentrations. K _d values were calculated with the manufacturer’s software using a global fit for both experiments.