Figure 6. PIN2 is essential for nitrate regulation of primary root growth and lateral root density.

1. Primary root length of Col‐0 wild‐type plants or eir1‐1 mutant plants was measured using the ImageJ program 3 days after 5 mM KNO₃ or KCl treatments. Tukey box plot show results from 3 independent biological replicates per experimental condition (n = 10–15 roots each replicate). The box plot shows the data within the interquartile range (25^th and 75^th percentiles) and a solid black line represents the median. Whiskers show maximum and minimum values no further than 1.5× IQR (interquartile range). Outlier data are plotted individually. Asterisk indicates statistically significant difference between analyzed by unpaired, two‐tailed and assuming equal variance t‐test (**P < 0.01).
2. Number of initiating and emerging lateral roots of Col‐0 or eir1‐1 mutant plants were counted using light microscopy 3 days after 5 mM KNO₃ or KCl treatments. Tukey box plot show results from 3 independent biological replicates per experimental condition (n = 10–15 roots each replicate). The box plot shows the data within the interquartile range (25^th and 75^th percentiles) and a solid black line represents the median. Whiskers show maximum and minimum values no further than 1.5× IQR (interquartile range). Asterisk indicates statistically significant difference between analyzed by unpaired, two‐tailed and assuming equal variance t‐test (**P < 0.01).

Source data are available online for this figure.