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. 2021 Jul 28;17(7):e10125. doi: 10.15252/msb.202010125

Figure EV1. Related to [Fig 1]: IMAP‐MS in primary macrophages and T cells.

Figure EV1

  1. Overview of MIP‐APMS experiments performed to study TLR2 activation and drug perturbation of MAPK14 in human immune cells.
  2. Lentiviral transduction efficiency of pLOC‐MAPK14 in U937 cells after antibiotic selection (2 d) determined by the GFP fluorescence of IRES‐GFP. Images represent three replicate experiments. Bar represents median % GFP‐positive cells from 3 experiments, error bars s.d.
  3. Workflow for IMAP‐MS in primary human macrophages.
  4. Interactome of MAPK14 and MAP3K7 in primary human macrophages (measured in quadruplicates) compared against each other.
  5. Interactome of MAPK14 in primary human T4 cells (measured in quadruplicates) compared against each other.