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. 2021 Jul 28;17(7):e10125. doi: 10.15252/msb.202010125

Figure 4. ARHGEF18 and FOSB are functional regulators downstream of TLR2.

Figure 4

  1. Volcano plot representing the interactome of MAP3K7 (measured 16× in biological replicates) compared against all other pull‐downs in the control group. The results of the t‐tests are represented in volcano plots, which show the protein enrichment versus the significance of the enrichment. Numbers indicate enrichment ranks with the heatmap labels of (C) serving as the legend. Significant interactors of MAP3K7 (two‐tailed t‐test, FDR < 0.01, enrichment > 4) are colored in blue (novel interactors) and green (known interactors).
  2. Interactors of MAP3K7 (blue: novel interactors, green: known interactors) with interconnecting proteins between different baits colored in gray.
  3. Heatmap of significant interactors of MAP3K7 upon activation, with significant hits in at least one time point (t‐test, P‐value < 0.05) denoted with an asterisk. Cell activation was performed for 5, 15, and 30 min with the TLR2 ligand PAM3CSK4 (P3C4).
  4. Heatmap of MAP3K7 PTMs (phosphorylation) upon activation, with significant hits (t‐test, P‐value < 0.05) in at least one time point denoted with an asterisk.
  5. Induction of NFκB determined based on luciferase luminescence in U937 NFκB reporter cells with CRISPR‐Cas9 knockouts of the potential novel interactors of MAP3K7 upon TLR2 activation (each bar represents a mean of four independent measurements; error bars represent the standard deviation; P‐values were calculated by t‐test. Asterisks indicate significant differences. ***P‐value < 0.001, **P‐value < 0.01).

Data information: Gray boxes indicate missing values. See also Appendix Figs S1–S14, Tables EV1 and EV2.