Table 1.
Primary and secondary antibodies for Western blot and immunofluorescence staining
| Antibody | Provider | Host | Catalog number | Dilution for WB | Dilution for IF |
|---|---|---|---|---|---|
| BIP | Affinity | Rabbit | AF5366 | 1:1000 | 1:150 |
| p-IRE1 | Affinity | Rabbit | AF7150 | 1:500 | 1:150 |
| p-PERK | Affinity | Rabbit | DF7576 | 1:500 | 1:150 |
| ATF6 | Affinity | Rabbit | DF6009 | 1:1000 | 1:150 |
| XBP1 | Proteintech | Rabbit | 25997-1-AP | 1:1000 | |
| p-eIF2a | Affinity | Rabbit | AF3087 | 1:500 | |
| eIF2a | Proteintech | Rabbit | 11170-1-AP | 1:1000 | |
| IRE | Proteintech | Rabbit | 27528-1-AP | 1:1000 | |
| PERK | Proteintech | Rabbit | 20582-1-AP | 1:1000 | |
| p-JNK | CST | Rabbit | #4671 | 1:500 | |
| JNK | CST | Rabbit | #9251 | 1:500 | |
| p-p38 | CST | Rabbit | #9216 | 1:500 | |
| p38 | CST | Rabbit | #14451 | 1:500 | |
| GAPDH | Proteintech | Mouse | 60004-1-Ig | 1:1000 | |
| Tublin | Proteintech | Mouse | 66031-1-Ig | 1:1000 | |
| NeuN | Abcam | Mouse | Ab104224 | 1:200 | |
| IBA1 | Abcam | Goat | Ab5076 | 1:200 | |
| GFAP | Abcam | Mouse | MAB360 | 1:200 | |
| Anti-rabbit IgG HRP | Aspen | Goat | AS1107 | 1:4000 | |
| Anti-mouse IgG HRP | Aspen | Goat | AS1106 | 1:4000 | |
| Alexa Fluor 488-AffiniPure anti-mouse IgG | Jackson | Goat | 115-545-003 | 1:500 | |
| Alexa Fluor 594-AffiniPure anti-rabbit IgG | Jackson | Goat | 111-585-003 | 1:500 | |
| Alexa Fluor 488-AffiniPure anti-goat IgG | Jackson | Donkey | 705-545-003 | 1:500 | |
| Alexa Fluor 594-AffiniPure anti-rabbit | Jackson | Donkey | 711-585-152 | 1:500 |
Abbreviations: BIP, binding immunoglobulin protein; IRE1, inositol-requiring kinase 1; PERK, protein kinase RNA-like endoplasmic reticulum kinase; ATF6, activating transcription factor 6; XBP1, X-box-binding protein 1; eIF2α, eukaryotic initiation factor 2α; JNK, c-jun NH2-terminal kinase; IBA1, ionized calcium-binding adapter molecule 1; NeuN, neuronal nuclei; GFAP, glial fibrillary acidic protein; HRP, horseradish peroxidase