FIG 4.

Potency of aminoglycosides against typical Mtb subpopulations found in lesions. (A) Aminoglycoside growth inhibitory activity against intracellular Mtb in THP-1-derived macrophages. Intracellular bacterial burden is shown for treated and drug-free control samples after 3 days of incubation at the concentrations indicated; D0, bacterial burden before drug treatment; D3 dmso, drug-free control on day 3. The experiment was performed twice with technical triplicates; one representative experiment is shown. (B) Bactericidal activity of aminoglycosides against nonreplicating Mtb persisters in ex vivo rabbit caseum (53). The red dotted line indicates the one-log kill mark or caseum MBC₉₀.