TABLE 3.

Primer sets for generating recombinant HSV-1 viruses that have a point mutation and for the respective revertant viruses

Strain	Mutation site		Primer
Mutant	UL5	K365N	Forward, 5′-cgagcacgagttcggtaacctcatgaatgtgctggagtacggcctgcccatcaccgaggatgacgacgataagta-3′
			Reverse, 5′-ctgcatgtgctcctcggtgatgggcaggccgtactccagcacattcatgaggttcaaccaattaaccaattctgattag-3′
		G352C	Forward, 5′-aacaacaaacggtgcgtcgagcacgagttctgtaacctcatgaaggtgctggagtacgaggatgacgacgataagtaggg-3′
			Reverse, 5′-cggtgatgggcaggccgtactccagcaccttcatgaggttacagaactcgtgctccaaccaattaaccaattctgattag-3′
		G352V	Forward, 5′-ccatttttattaacaacaaacggtgcgtcgagcacgagttcgttaacctcatgaaggtaggatgacgacgataagtaggg-3′
			Reverse, 5′-aggccgtactccagcaccttcatgaggttaacgaactcgtgctcgacgcaccgttcaaccaattaaccaattctgattag-3′
		M355T	Forward, 5′-ccatttttattaacaacaaacggtgcgtcgagcacgagttcggtaacctcacgaaggtaggatgacgacgataagtaggg-3′
			Reverse, 5′-aggccgtactccagcaccttcgtgaggttaccgaactcgtgctcgacgcaccgttcaaccaattaaccaattctgattag-3′
		E34K	Forward, 5′-accgaccagccgttccagggagggccaaggcctttttaaattttacgtctatgcacggaggatgacgacgataagtaggg-3′
			Reverse, 5′-ggattggctgcaccccgtgcatagacgtaaaatttaaaaaggccttggccctcccaaccaattaaccaattctgattag-3′
		S314I	Forward, 5′-accagaaactgcggtgttccgtccgccagatcgagaacgtgctcacgtacctcatctgaggatgacgacgataagtaggg-3′
			Reverse, 5′-cgcagcgtgcggttgcagatgaggtacgtgagcacgttctcgatctggcggacggcaaccaattaaccaattctgattag-3′
		G477D	Forward, 5′-cgaataccgacggctgacacaccagcccgacctgactattgaaaagtggctcacggccaggatgacgacgataagtaggg-3′
			Reverse, 5′-gatgcggctggcgttggccgtgagccacttttcaatagtcaggtcgggctggtgtcaaccaattaaccaattctgattag-3′
	UL52	F360V	Forward, 5′-accgtgaattcattacgtacatctacctggcccatgttgagtgtttcagccccccgcgaggatgacgacgataagtaggg-3′
			Reverse, 5′-agatgcgtggctaggcgcggggggctgaaacactcaacatgggccaggtagatgtcaaccaattaaccaattctgattag-3′
		F360C	Forward, 5′-accgtgaattcattacgtacatctacctggcccattgtgagtgtttcagccccccgcgaggatgacgacgataagtaggg-3′
			Reverse, 5′-agatgcgtggctaggcgcggggggctgaaacactcacaatgggccaggtagatgtcaaccaattaaccaattctgattag-3′
		N902T	Forward, 5′-atgtgagcttctttgaaaggaaggcgtcccgcaccgcgctggaacactttgggcgacgaggatgacgacgataagtaggg-3′
			Reverse, 5′-tccgtcagggtctcgcgtcgcccaaagtgttccagcgcggtgcgggacgccttcccaaccaattaaccaattctgattag-3′
		S364G	Forward, 5′-cattacgtacatctacctggcccattttgagtgtttcggccccccgcgcctagccacgaggatgacgacgataagtaggg-3′
			Reverse, 5′-cacggcccgaagatgcgtggctaggcgcggggggccgaaacactcaaaatgggcccaaccaattaaccaattvtgattag-3′
		V222C	Forward, 5′-gcgcgtgcttgccgcgtaccgcagggcgtattgtggaagcgcgcagagtcccttctggaggatgacgacgataagtaggg-3′
			Reverse, 5′-gaatttgctaagaaaccagaagggactctgcgcgcttccacaatacgccctgcggcaaccaattaaccaattctgattag-3′
		F294L	Forward, 5′-ccccccgccccgacaccgtcagcgctgcgtccctgacctcgcttgccgccatcacgaggatgacgacgataagtaggg-3′
			Reverse, 5′-cgtgcaacagaaccgcgtgatggcggcaagcgaggtcagggacgcagcgctgacgcaaccaattaaccaattctgattag-3′
Revertant	UL5	352-356	Forward, 5′-aacaacaaacggtgcgtcgagcacgagttcggtaacctcatgaaggtgctggagtacgaggatgacgacgataagtaggg-3′
			Reverse, 5′-cggtgatgggcaggccgtactccagcaccttcatgaggttaccgaactcgtgctccaaccaattaaccaattctgattag-3′
	UL52	F360V/C	Forward, 5′-accgtgaattcattacgtacatctacctggcccattttgagtgtttcagccccccgcgaggatgacgacgataagtaggg-3′
			Reverse, 5′-agatgcgtggctaggcgcggggggctgaaacactcaaaatgggccaggtagatgtcaaccaattaaccaattctgattag-3′
		N902T	Forward, 5′-atgtgagcttctttgaaaggaaggcgtcccgcaacgcgctggaacactttgggcgacgaggatgacgacgataagtaggg-3′
			Reverse, 5′-tccgtcagggtctcgcgtcgcccaaagtgttccagcgcgttgcgggacgccttcccaaccaattaaccaattctgattag-3′