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. 2021 Sep 17;65(10):e00507-21. doi: 10.1128/AAC.00507-21

FIG 3.

FIG 3

The specific interaction between IMP-1, mediated by Lys87, Lys89, Lys145, and Lys147, and the bacterial membrane promotes its packaging into vesicles. (A) SDS-PAGE analysis of OMVs from E. coli expressing IMP-1 and IMP-1 4KE mutant (IMP-1 K87E/K89E/K145E/K147E). (B) Immunoblot analysis of OMVs and whole cells (WC) from E. coli expressing IMP-1 or IMP-1 4KE. (C) Comparison between levels of WT IMP-1 and IMP-1 4KE into OMVs. The plotted values, relativized to the IMP-1 value, were obtained as described in Materials and Methods. Data correspond to three independent experiments and are shown as the mean value. Error bars represent the standard deviation (SD). (D) Imipenem hydrolysis rate by OMVs purified from E. coli expressing IMP-1 or IMP-1 4KE. Data correspond to two independent experiments and are shown as the mean value. Error bars represent SD.