TABLE 1.
Strains used in this study
| Strain | Description | Source or reference |
|---|---|---|
| W3110 | Wild-type Escherichia coli, F−, λ− | NEB |
| WJW00 | Derived from W3110 by deleting gmhD; can only synthesize Kdo2-lipid A | 30 |
| WJW01 | Derived from W3110 by deleting rfa operon (14 genes); can only synthesize Kdo2-lipid A | This study |
| WJW02 | Derived from WJW01 by deleting wbbL-rfbB operon (11 genes); can only synthesize Kdo2-lipid A | This study |
| WJW03 | Derived from WJW02 by deleting galF-wza operon (21 genes); cannot synthesize colanic acid | This study |
| WJW04 | Derived from WJW03 by deleting yccC-ymcD operon (7 genes); cannot synthesize type IV EPS | This study |
| WJW05 | Derived from WJW04 by deleting flhE-motA operon (13 genes); cannot synthesize flagella | This study |
| WJW06 | Derived from WJW05 by deleting flagellar operon fliY-fliR (30 genes) | This study |
| WJW07 | Derived from WJW06 by deleting flagellar operon flgN-flgL (14 genes) | This study |
| WJW08 | Derived from WJW07 by deleting fimbrial operon fimB-fimH (9 genes) | This study |
| WJW09 | Derived from W3110 by deleting EPS operons galF-wza and yccC-ymcD (28 genes) | This study |
| WJW010 | Derived from W3110 by deleting flagellar operons flhE-motA, fliY-fliR, and flgN-flgL (57 genes) | This study |
| WJW011 | Derived from WJW01 by deleting fimB-fimH operon (9 genes); cannot synthesize fimbria | This study |
| ΔwaaFwaaL-Q | Derived from W3110 by deleting waaF and waaL-waaQ operon (11 genes); can synthesize Hep-Kdo2-lipid A which contains a single heptose | This study |
| ΔwaaC | Derived from W3110 by deleting waaC; can only synthesize Kdo2-lipid A | 31 |