Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Plasmid map of pISApl1-CRISPR/Cas9 and the transposon Tn::ISApl1/CRISPR/Cas9. (a) Plasmid pISApl1-CRISPR/Cas9 containing the cas9 gene with the promoter pLteto-1 (39) and the sgRNA with the synthetic J23119 promoter; F and R denote the locations of primers used for the sgRNA construct. (b) The Tn::ISApl1-CRISPR/Cas9, the cas9, and the tpm gene, along with sgRNA was flanked by two ISApl1 in the same orientation.
