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Characterization of escape mutants that tolerated transformation of the pISApl1-CRISPR/Cas9 construct. (a) The CRISPR/Cas9 region of the escape mutants was amplified by a high-fidelity enzyme and followed by sanger sequencing. (b, c) The results show that 510 or 511 deletions at the same sgRNA area led to pISApl1-CRISPR/Cas9 inactivation in the successful transformants.
