Fig 3. Screening of toothpaste and mouthwash ingredients exhibited inhibitory effects on the serine protease activity of TMPRSS2.

Eighteen toothpaste and mouthwash ingredients at 1% (w/w) exhibited inhibitory effects on the TMPRSS2 serine protease activity. TMPRSS2 cleaved Boc-Gln-Ala-Arg-MCA as the substrate and produced the potent fluorophore, AMC (7-amino-4-methylcoumarin). Values were normalized against the intensity of the absence of test ingredients. Data are expressed as the mean ± SD (n = 3).