Fig 1. pH- and LAMP1-dependence of LASV pseudovirus infection.
(A) Human HEK2393T or A549 cells transduced with control (shScr) or LAMP1-specific shRNA (shLAMP1) were infected with luciferase-encoding HIV-1 particles pseudotyped with LASV GPC (LASVpp) or Influenza A HA/NA glycoproteins (IAVpp). Control infections were done in the presence of 30 mM NH4Cl to block virus entry from endosomes. The resulting luciferase signal was measured at 48 hpi and plotted as mean and SEM from three independent experiments performed in duplicate. (B) Western blot analysis of LAMP1 expression and the efficiency of shRNA knockdown in HEK293T and A549 cells. Densitometry analysis of LAMP1 expression levels relative to control cells is shown under the bottom panel. Anti-tubulin served as the loading control. (C) LASVpp infection of avian QT6 cells transfected with an empty vector or human LAMP1-mRFP encoding plasmid. Data are mean and SD for two independent experiments performed in triplicate. *, p < 0.05, **, p < 0.01, ***, p < 0.001, NS, not significant.